GetterDragun wrote:He killed one of the original cone heads in the G1 movie, it was just cut from it.
As I recall, he gut-punched Dirge, flipped him over, and drove him head first into the ground. I don't recall whether Prime was supposed to have killed Dirge, though.
You could make a case for Prime having indirectly killed Kickback, Shrapnel, and Thundercracker in TF:TM. Shrapnel was smacked by Prime in truck mode and then presumably gunned down along with Kickback and TC (and various others that didn't seem to be damaged nearly as much by Prime's shots). While the shots weren't fatal (at least not immediately), they did result in a mid-flight ejection for those three.
Prime shoots at various unseen Decepticons as well; it's possible he hit Skywarp too. (Bombshell was nearly blown up by Springer when he was lobbing mortars at Devastator, and is the only ejected Decepticon that apparently avoided a Prime beating.)
And of course there's the beating he gave Megatron, that resulted in possibly-fatal injuries.